Journal: Microorganisms

Article Title: A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs

doi: 10.3390/microorganisms11112758

Figure Lengend Snippet: Sensitivity and specificity of the SrP-iELISA, QrP-iELISA, and commercial ELISA (IDvet) used to detect antibodies against African swine fever virus (ASFV) in this study.

Article Snippet: ASFV infection was confirmed using a commercial real-time PCR kit, the VDx ® ASFV qPCR kit (Median Diagnostics Inc., Chuncheon, Gangwon-do, Republic of Korea).

Techniques: Enzyme-linked Immunosorbent Assay, Virus

Journal: Microorganisms

Article Title: A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs

doi: 10.3390/microorganisms11112758

Figure Lengend Snippet: Comparison of antibody responses to SrP and QrP in experimentally infected pig serum samples. ( a ) Comparison of QrP-ELISA and IDvet results using serum samples of pigs infected with ASFV I. ( b ) Comparison of QrP-ELISA and IDvet results using serum samples of pigs infected with ASFV II. ( c ) Comparison of SrP-ELISA results using serum samples of pigs infected with ASFV I. ( d ) Comparison of SrP-ELISA results using serum samples of pigs infected with ASFV II. The X -axis of each plot shows the days post-infection (DPI), and the Y -axis shows the OD 450 value. The horizontal dashed lines show the OD 450 threshold for positivity.

Article Snippet: ASFV infection was confirmed using a commercial real-time PCR kit, the VDx ® ASFV qPCR kit (Median Diagnostics Inc., Chuncheon, Gangwon-do, Republic of Korea).

Techniques: Comparison, Infection, Enzyme-linked Immunosorbent Assay

Journal: Microorganisms

Article Title: A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs

doi: 10.3390/microorganisms11112758

Figure Lengend Snippet: Differences in the antibody response in SrP-ELISAs, QrP-ELISA, and commercial kit according to DPI.

Article Snippet: ASFV infection was confirmed using a commercial real-time PCR kit, the VDx ® ASFV qPCR kit (Median Diagnostics Inc., Chuncheon, Gangwon-do, Republic of Korea).

Techniques: Infection

Journal: Microorganisms

Article Title: Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

doi: 10.3390/microorganisms13030615

Figure Lengend Snippet: PCR Programs for Different Multiplex Reactions.

Article Snippet: Numerous commercial PCR kits are available, including the INgene qPPA (Gold Standard Diagnostics, Davis, CA, USA), ASFV virus type (Indical Bioscience, Leipzig, Germany), ID Gene ASF Duplex (IDvet, Grabels, France), RealPCR ASFV (IDEXX, Westbrook, ME, USA), SwineFever combi (Gerbion, Kornwestheim, Germany), ViroReal kit ASFV virus (Ingenetix, Wien, Austrilia), and Kylt ASF Real-Time PCR kits (Anicon, Höltinghausen, Germany) [ ].

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction

Journal: Microorganisms

Article Title: Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

doi: 10.3390/microorganisms13030615

Figure Lengend Snippet: Analytical Sensitivity of Quadplex Real-Time PCR with Different Multiplex Reaction Buffers Using Plasmid-spiked Samples.

Article Snippet: Numerous commercial PCR kits are available, including the INgene qPPA (Gold Standard Diagnostics, Davis, CA, USA), ASFV virus type (Indical Bioscience, Leipzig, Germany), ID Gene ASF Duplex (IDvet, Grabels, France), RealPCR ASFV (IDEXX, Westbrook, ME, USA), SwineFever combi (Gerbion, Kornwestheim, Germany), ViroReal kit ASFV virus (Ingenetix, Wien, Austrilia), and Kylt ASF Real-Time PCR kits (Anicon, Höltinghausen, Germany) [ ].

Techniques: Real-time Polymerase Chain Reaction, Multiplex Assay, Plasmid Preparation

Journal: Microorganisms

Article Title: Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

doi: 10.3390/microorganisms13030615

Figure Lengend Snippet: Evaluation of Quadplex Real-time PCR Sensitivity and Specificity Compared to Standard Singular Real-time PCR Assay in Serum Samples from Experimentally Infected Pigs.

Article Snippet: Numerous commercial PCR kits are available, including the INgene qPPA (Gold Standard Diagnostics, Davis, CA, USA), ASFV virus type (Indical Bioscience, Leipzig, Germany), ID Gene ASF Duplex (IDvet, Grabels, France), RealPCR ASFV (IDEXX, Westbrook, ME, USA), SwineFever combi (Gerbion, Kornwestheim, Germany), ViroReal kit ASFV virus (Ingenetix, Wien, Austrilia), and Kylt ASF Real-Time PCR kits (Anicon, Höltinghausen, Germany) [ ].

Techniques: Real-time Polymerase Chain Reaction, Infection, Injection

Journal: Microorganisms

Article Title: Specific Detection of African Swine Fever Virus Variants: Novel Quadplex Real-Time PCR Assay with Internal Control

doi: 10.3390/microorganisms13030615

Figure Lengend Snippet: Validation of the Quadplex Real-time PCR with Field Samples.

Article Snippet: Numerous commercial PCR kits are available, including the INgene qPPA (Gold Standard Diagnostics, Davis, CA, USA), ASFV virus type (Indical Bioscience, Leipzig, Germany), ID Gene ASF Duplex (IDvet, Grabels, France), RealPCR ASFV (IDEXX, Westbrook, ME, USA), SwineFever combi (Gerbion, Kornwestheim, Germany), ViroReal kit ASFV virus (Ingenetix, Wien, Austrilia), and Kylt ASF Real-Time PCR kits (Anicon, Höltinghausen, Germany) [ ].

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction

Journal: bioRxiv

Article Title: Direct colorimetric LAMP assay for in-field detection of African swine fever virus: a validation study during an outbreak in Vietnam

doi: 10.1101/2020.06.03.132944

Figure Lengend Snippet: A) The amplified product was visualized by the color change from red to yellow (positive signal) and pink (negative signal). One ng of synthesized DNA template was used. The reactions were incubated at 60 °C for 30 min. B) LAMP product was analyzed by agarose gel electrophoresis and compared with the routine PCR result. Abbreviation, M: DNA ladder.

Article Snippet: Genomic DNA of ASFV was identified by a commercial real-time PCR kit (Median Diagnostics Inc., http://www.mediandiagnostics.com/asfv.php ).

Techniques: Amplification, Synthesized, Incubation, Agarose Gel Electrophoresis

Journal: Viruses

Article Title: Assessment of Nine Real-Time PCR Kits for African Swine Fever Virus Approved in Republic of Korea

doi: 10.3390/v16101627

Figure Lengend Snippet: The various diagnostic methods for African swine fever virus (ASFV).

Article Snippet: 7 , Optipharm (Cheongju-si, Republic of Korea) , Opti ASFV qPCR Kit , 96 , 238-002 , 8 September 2020.

Techniques: Diagnostic Assay, Virus

Journal: Viruses

Article Title: Assessment of Nine Real-Time PCR Kits for African Swine Fever Virus Approved in Republic of Korea

doi: 10.3390/v16101627

Figure Lengend Snippet: Information on the African swine fever virus real-time PCR diagnostic kits approved by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) in the Republic of Korea.

Article Snippet: 7 , Optipharm (Cheongju-si, Republic of Korea) , Opti ASFV qPCR Kit , 96 , 238-002 , 8 September 2020.

Techniques: Virus, Real-time Polymerase Chain Reaction, Diagnostic Assay

Journal: Viruses

Article Title: Assessment of Nine Real-Time PCR Kits for African Swine Fever Virus Approved in Republic of Korea

doi: 10.3390/v16101627

Figure Lengend Snippet: The specificity results of nine real-time PCR kits approved by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) in the Republic of Korea for detecting African swine fever virus (ASFV). RFU, relative fluorescence unit; PCV2, Porcine circovirus 2; PPV, Porcine Parvovirus ; PPrV, Porcine Pseudorabies virus .

Article Snippet: 7 , Optipharm (Cheongju-si, Republic of Korea) , Opti ASFV qPCR Kit , 96 , 238-002 , 8 September 2020.

Techniques: Real-time Polymerase Chain Reaction, Virus, Fluorescence